Strain Engineering for Adipic Acid

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CD Biosynsis uses renewable resources to synthesize adipic acid through metabolic engineering and optimization of fermentation conditions and is committed to exploring ways to increase the yield of adipic acid.

Background

Adipic acid is an aliphatic dicarboxylic acid containing 6 carbons. Adipic acid is naturally found mainly in human urine, and its presence in plants and microorganisms has not been reported. Adipic acid has been widely used as a monomeric raw material for the production of polymers such as nylon, chemical fibers, and engineering plastics in the food, pharmaceutical, and plastic industries. Currently, the main production method for adipic acid is nitric acid oxidation using a mixture of cyclohexanol and cyclohexanone as raw materials. However, the strong oxidizing nitric acid in the nitric acid oxidation process causes severe corrosion of the equipment and generates nitrogen oxides as a by-product, which causes great environmental pollution. Therefore, the direct production of adipic acid using microorganisms has become a major concern for synthetic biologists.

Figure 1. Reverse β-oxidation pathway for adipic acid biosynthesis from TCA metabolites. (Sengupta S, et al., 2021)

What We Provide

Our scientists have constructed adipic acid-related synthetic pathways in microorganisms based on biosynthetic strategies to achieve green and sustainable adipic acid production through biosynthesis.

Featured Services

We can achieve de novo synthesis of adipic acid in microorganisms.
We are able to help our customers with the construction and screening of high-copy substrates for the reverse degradation pathway of adipic acid.
We are able to modify the rate-limiting enzymes in the adipic acid synthesis pathway.
We are able to help customers obtain high yield strains of adipic acid using genome editing techniques.
We can design protein scaffolds to anchor the enzymes involved in adipic acid synthesis to the protein scaffold to increase the yield of the target product.

Key Technologies

Deliverables

Adipic acid-producing microorganisms.
Adipic acid.

How We Can Help

Optimization of Adipic Acid Synthesis Pathway

Microorganisms can synthesize adipic acid directly from a variety of edible raw materials. We can provide a variety of metabolic engineering modification strategies to help our customers improve the efficiency of adipic acid production. We are able to provide optimization services for the following adipic acid synthesis pathways.

Optimization of the ω-oxidation pathway. We can optimize the three stages of the ω-oxidation pathway using a module optimization strategy. We improve the yield of adipic acid by regulating the expression levels of genes related to the hydroxylation and oxidation modules, respectively.
Optimizing the β-oxidation pathway. We can overexpress key genes in the β-oxidation pathway or construct cofactor regeneration systems to increase the yield of glycolic acid.

Construction of a Novel Synthetic Pathway for Adipic Acid

Adipic acid can be degraded to succinyl coenzyme A and acetyl coenzyme A in microorganisms, and our scientists are working to design the inverse adipic acid degradation pathway to achieve the synthesis of glycolic acid. If you would like to join us in exploring synthetic pathways for inverse adipic acid degradation in microorganisms, please contact us directly.

Construction of a Semi-Synthetic Pathway for Adipic Acid

We have successfully constructed a semi-synthetic pathway for the production of adipic acid from glucose. We use microbial fermentation to produce glucaric acid and then prepared adipic acid chemically using the monopotassium salt of glucaric acid as the starting reactant.

Applications of Adipic Acid

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive adipic acid production and meet the needs of customers in a variety of industries.

As a food additive.
As a flavoring agent.
As a dye.
As a spice.
Used in the production of nylon.
Used in the production of chemical fibers.
Used in the production of engineering plastics.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in adipic acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

Shin JH, et al. Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid. Microb Cell Fact. 2021 Aug 4; 20(1): 155.

Please note that all services are for research use only. Not intended for any clinical use.

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