Strain Engineering for Resveratrol

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CD Biosynsis is dedicated to optimizing plant cell chassis and microbial cell chassis through genetic engineering, metabolic engineering, and protein engineering technologies to enhance resveratrol production.

Background

Resveratrol is a secondary metabolite that is naturally synthesized in a variety of plants. Due to the important pharmacological effects of resveratrol, with anti-inflammatory, antioxidant, anti-aging and anti-diabetic effects, the large-scale production of resveratrol has become inevitable. However, resveratrol is produced in very small quantities in plants and is susceptible to seasonal limitations that do not meet the commercial demand for it in various fields. Therefore, it is of great value to investigate synthetic biology methods that can increase the yield of resveratrol.

Figure 1. Glutarate biosynthesis via lysine catabolism. (Li W, et al., 2019) Figure 1. De novo synthesis pathway of resveratrol in recombinant strain E. coli. (Liu X, et al., 2016)

What We Provide

Based on the synthetic biology platform, we are able to optimize plant cell chassis and microbiological chassis for resveratrol production based on metabolic engineering, protein engineering, and microbial engineering strategies.

Genetic Engineering

We are able to provide customized services using transgenic technology to enable non-resveratrol producing species to have the ability to synthesize resveratrol.

Metabolic Engineering

We are able to modify the resveratrol biosynthetic pathway through metabolic engineering tools to increase the resveratrol production capacity of chassis cells.

Plant Metabolic Engineering

Plants can be used as bioreactors for resveratrol synthesis. We are able to assist our customers in screening suitable plant chassis or modulating metabolic fluxes in the resveratrol synthesis pathway to achieve resveratrol production at scale.

Protein Engineering

We are able to use protein engineering tools to increase the production capacity of resveratrol in chassis cells through strategies such as modifying key enzymes in the resveratrol synthesis pathway or constructing cell-free expression systems.

Deliverables

Efficient plant cell factory for resveratrol production.
Efficient microbial cell factory for resveratrol production.
High quality resveratrol.

How We Can Help

Development of Synthetic Biology Chassis for Resveratrol Production

We are able to develop plant, bacterial and fungal chassis for resveratrol production. We are able to help our customers screen suitable host organisms according to their needs. The following are the chassis that has been used for the production of resveratrol, and we are able to provide modification services for these microbial chassis. Please contact us directly if you have other chassis of interest for resveratrol production.

Pseudomonas putida	Escherichia coli	Nicotiana tabacum	Saccharomyces cerevisiae
Aspergillus	Botryosphaeria	Penicillium	Fusarium
Alternaria	Arcopilus	Lasiodiplodia	Yarrowia lipolytica

Resveratrol Production Using Plant Cell Suspensions

We are able to culture isolated plant cells in a liquid medium using the plant cell suspension culture technique. We help our customers to establish suitable cell suspension culture systems to increase the yield of resveratrol and shorten the production cycle.

Construction of Co-Culture System for Resveratrol Production

We are able to construct a co-culture system to improve the yield of resveratrol by optimizing fermentation parameters, changing the inoculation ratio of co-culture strains, incubation temperature and time, and other strategies.

Development of Resveratrol-Producing Endophytic Fungi

Endophytic fungi in symbiosis with plants are able to gain access to the secondary metabolite production of plants. We can help our customers to screen endophytic fungi for resveratrol production and optimize the metabolic pathways in endophytic fungi to increase the concentration of resveratrol accumulation.

Applications of Resveratrol

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive resveratrol production and meet the needs of customers in a variety of industries.

For the treatment of diabetes.
For the treatment of cardiovascular diseases.
For the treatment of neurological disorders.
For cancer therapy.
As an additive in yogurt.
As an ingredient in skincare and beauty products.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in resveratrol production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

Liu X, et al. De novo biosynthesis of resveratrol by site-specific integration of heterologous genes in Escherichia coli. FEMS Microbiol Lett. 2016 Apr; 363(8): fnw061.
Pannu N, Bhatnagar A. Resveratrol: from enhanced biosynthesis and bioavailability to multitargeting chronic diseases. Biomed Pharmacother. 2019 Jan; 109: 2237-2251.

Please note that all services are for research use only. Not intended for any clinical use.

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