Strain Engineering for Ribosomal Peptide

CD Biosynsis is committed to helping our customers develop synthetic biology strategies to create cost-effective ways to produce ribosomal peptides and break the limitations of ribosomal peptides in traditional production processes. Our expertise in synthetic biology chassis development and metabolic engineering can help our customers overcome all difficulties in the production and application of ribosomal peptides.

Advantages of Synthetic Biology-Driven Ribosomal Peptide Production

Ribosomal peptides or ribosomally synthesized and post-translationally modified peptides (RiPPs) are a large group of natural products synthesized from ribosomes and post-translationally modified, with a wide range of structural and biological activity diversity, which have a wide range of applications in the food industry, pharmaceutical industry, and agriculture fields. Traditional methods for the production of ribosomal peptides are usually based on the solid-phase synthesis, liquid-phase synthesis, and in vivo biotechnological recombination techniques, but this approach possesses many limitations, such as high production costs and low purity of the resulting products. The revolutionary technologies and innovative engineering concepts of synthetic biology have opened a new avenue for the development of microbial platforms for the production of Ribosomal Peptides. Moreover, the industrialization of synthetic biology-driven microbial production processes is an effective way to improve the functional properties of Ribosomal Peptides and reduce the production cost of Ribosomal Peptides.

Figure 1. Peptide bioengineering strategies. (Huang HM, et al., 2019)

What We Provide

CD Biosynsis leverages our advanced technologies in bacterial synthetic biology, fungal synthetic biology, enzyme engineering, genetic engineering, and metabolic engineering to help our customers overcome the challenges of ribosomal peptide production. Our exceptional scientists can provide customized solutions to meet our customers' specific needs for ribosomal peptide production and applications.

Deliverables

• Superior ribosomal peptide-producing microorganisms.
• High-quality ribosomal peptide products, including lanthipeptides, thiopeptides, sactipeptides, etc.

How We Can Help

Heterologous Production of Ribosomal Peptides

We can help our customers design and develop microbial cell factories and reconfigure biosynthetic pathways in the heterologous hosts through the powerful synthetic biology-based genetic manipulation platform and computer-aided technologies for large-scale, efficient ribosomal peptide production. The following is a list of microbial strains that can be used for the heterologous production of ribosomal peptides. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.

Artificial Evolution of Ribosomal Peptides Biosynthetic Pathways

Our scientists have established synthetic promoter libraries to reconstitute biosynthetic pathways for the production of specific ribosomal enzymes based on high-throughput screens. We have optimized the expression of ribosomal peptides by establishing a rational combination strategy of promoters with different intensities to improve the yield of ribosomal peptides.

Applications of Ribosomal Peptides

CD Biosynsis leverages our specialized synthetic biology platform and extensive technical experience to customize synthetic biology-driven ribosomal peptide production solutions for our customers to meet the growing needs in the following areas.

• Used in the synthesis of peptide antibiotics.
• Used in the development of anti-tumor drugs.
• Used in the development of anti-HIV drugs.
• Used in the manufacture of detergents.
• Used in the suppression of plant diseases.
• Used in food industry production.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in protein and peptide production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

1. Wu Z, et al. Microbial production of small peptide: pathway engineering and synthetic biology. Microb Biotechnol, 2021, 14(6): 2257-2278.
2. Tajbakhsh M, et al. Overview of ribosomal and non-ribosomal antimicrobial peptides produced by Gram positive bacteria. Cell Mol Biol (Noisy-le-grand), 2017, 63(10): 20-32.

Please note that all services are for research use only. Not intended for any clinical use.