Yeast Genome Editing Services

Overview Capabilities Advantages Services Process FAQs

Overview

Our company is committed to delivering the best yeast genome editing services to customers. We apply cutting-edge gene editing technologies including CRISPR/Cas9, TALENs and ZFNs to deliver precise and effective genome editing solutions for various yeast species. These services are used extensively in functional genomics, metabolic engineering, synthetic biology, and industrial biotechnology.

Applications of CRISPR/Cas gene-editing technology in yeast (B Liao, et al.,2022)

We edit your yeast genome in several ways, including CRISPR/Cas9 gene knock-in, homologous recombination, gene overexpression, site-directed mutations, and conditional gene expression. Such technologies enable scientists to accurately manipulate the yeast genome in order to learn about how genes work, to maximise metabolic pathways, and create specific yeast strains with unique traits.

Additionally, we also offer complete genomic integration analysis services that enable our customers to identify deep metabolic networks and optimise large-scale pathways in yeast by combining genomics, transcriptomics, proteomics and metabolomics data.

Capabilities

The yeast genome editing services provided by our company mainly include the following sub-services:

Service Type	Description	Application
Plasmid expression system	The use of plasmids to introduce and express genes in yeast provides flexible ways to engineer and optimize pathways.	Testing and optimizing metabolic pathways are often used for research and early development.
CRISPR/Cas9 gene editing	Precise and efficient genome editing using CRISPR/Cas9 technology allows targeted gene knockouts, insertions or modifications in yeast.	Functional genomics, metabolic engineering, synthetic biology, etc.
homologous recombination	Use yeast's natural DNA repair mechanisms to introduce precise genetic changes, including gene knockouts, insertions or substitutions.	Create specific genetic modifications, often used to build yeast strains with gene deletions or replacement.
Gene knockout/knockdown	Targeting the deletion or suppression of specific genes in yeast to study their function and role in cellular processes.	Study the function of individual genes, identify essential genes, and study gene-specific phenotypes.
gene overexpression	Introduce or amplify genes in yeast to enhance the activity of key enzymes or proteins and improve overall production or function.	Increase production throughput of biofuels, drugs and enzymes.
point mutation	Specific mutations were introduced at specific locations in the yeast genome to study the impact of these changes on gene function and protein activity.	Analyze the functional contribution of proteins, structure-function research.
conditional gene expression	Creating gene expression systems that can induce or suppress expression under specific conditions allows temporal control of gene function.	Study essential genes and understand the gene functions of yeast under different environmental conditions.
Synthetic pathway construction	Design and assemble anabolic pathways to introduce new biosynthetic capabilities or enhance existing capabilities.	Create new biosynthetic routes, often used in the production of complex drugs and specialty chemicals.

Advantages

Our yeast genome editing solutions come with these advantages:

Gene editing with high precision and efficiency

We use the cutting-edge technologies of CRISPR/Cas9, TALENs and ZFNs to deliver precise and efficient editing of the yeast genome for functional genomics, metabolic engineering, synthetic biology and industrial biotechnology fields.

Broad scope of application

Our services cover a variety of gene editing needs from gene knockouts, insertions, overexpressed site-directed mutations, and support research on yeast gene functions, optimization of metabolic pathways, construction of synthetic biological pathways, and development of industrial strains.

High-throughput screening capabilities

Our services support high-throughput screening and enable editing under specific genetic backgrounds and industrial conditions, simplifying the genome editing process for diploid wild/industrial yeast strains.

Flexible editing strategies

We provide a variety of editing strategies, including homologous recombination, conditional gene expression, and adaptive laboratory evolution. Editing plans can be customized according to specific needs to meet the needs of different research and industrial applications.

Comprehensive technical support and project management

From project consultation, editing tool design, carrier construction to yeast transformation, screening and verification, optimization amplification and report writing, we provide end-to-end professional services to ensure the smooth progress and successful delivery of projects.

Services Process

The service process of the Yeast Genome Editing Service is summarized as follows:

Project consultation

First of all, customers need to communicate with the company about technical solutions and clarify the needs and goals of technical services. This step includes understanding the customer's specific research needs and biotechnology application goals.

Editing tool design and construction

Design and build required editing tools based on customer needs, such as gene editing tools such as CRISPR/Cas9, TALENs or ZFNs. These tools will be used to accurately modify the yeast genome.

Vector construction

Using cloning technology to integrate editing tools into appropriate vectors to facilitate subsequent yeast transformation.

Yeast transformation

Transform the constructed vector into the target yeast strain, and achieve gene editing through steps such as PCR reaction setting, PCR product purification, and transformation of plasmid and HDR template DNA.

Screening and verification

Screening is performed among transformed yeast strains to determine which strains have successfully achieved gene editing. The editing results are then verified by PCR amplification, sequencing and other methods to ensure the accuracy and effectiveness of the editing.

Optimization and amplification

Optimize the editing process based on experimental results and expand the number of edited yeast strains to meet the needs of subsequent research or industrial applications.

Project delivery

After completing all experimental steps, the company will put sub-packaged samples into storage and write a detailed project report or composition analysis report. Finally, arrange product delivery and report delivery according to customer requirements.

After-sales support

After the project is delivered, the company will continue to provide technical support and after-sales follow-up services to ensure that customers can successfully use the edited yeast strain for further research or production.

Project consultation

Editing tool design and construction

Vector construction

Yeast transformation

Screening and verification

Optimization and amplification

Project delivery

After-sales support

FAQs

Below are frequently asked queries and responses about the services of yeast genome editing.

Q: What is yeast genome editing?

A: Yeast genome editing is the accurate and efficient editing of yeast cell genomes through gene knockouts, inserts or deletions. It is commonly applied in functional genomics, metabolic engineering, synthetic biology and industrial biotechnology.

Q: How are the technologies for editing yeast genomes?

A: Using cutting-edge technologies such as CRISPR/Cas9, TALENs and ZFNs, we edit yeast genomes so that the editing is accurate and reliable.

Q: What are the uses of yeast genome editing?

A: Genome editing can be employed to examine gene functions, regulate metabolic routes, create novel biological products and enhance the efficiency of industrial strains.

Q: What's the best editing approach?

A: Select an editing technique that best suits your needs. For instance, CRISPR/Cas9 can be used for fast, precise gene editing, while homologous recombination can be used for high-accuracy gene editing.

Q: How does yeast genome editing work?

A: Yeast genome editing can be used to research gene functions, improve metabolic pathways, create novel biological products, and boost the productivity of industrial strains.

Q: So how can editing achieve a high success rate?

A: We leverage a variety of tools to enhance editing performance, such as an optimized Cas9 system, efficient sgRNA designs and tailor-made editing solutions.

Q: What can be said about the accuracy of the corrected yeast strain?

A: We check edited yeast strains by PCR analysis, sequencing and functional experiments of various kinds in order to verify that the editing was indeed successful and reliable.

Q: Can experimental follow-up services be offered?

A: We offer post-treatment experimental services, like cell transfection/infection, in vivo injection, functional studies, etc. to satisfy customers of all types.

Q: What about acquiring corrected yeast strains?

A: Upon editing, we deliver the edited yeast strain and associated data to the customer and offer technical assistance and after-sales support.

Q: How much does yeast genome editing cost?

A: We provide affordable and competitive rates, based on the size of the project and the services you want. Contact our customer support to get a complete quote.

Q: Do you support high-throughput screening?

A: Yes, we encourage high-throughput screening and are able to perform large-scale screening experiments in an appropriate genetic environment and industry.

Q: How can I reach out to your company for an appointment or order?

A: You may submit the order form on our website for consultation or ordering. We have a technical support staff who can help you out.

Please note that all services are for research use only. Not intended for any clinical use.

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