CRISPR-based Gene Editing Services

Overview Capabilities Services Process Advantages FAQs

Overview

CD Biosynsis is dedicated to offering customers efficient, precise and complete gene editing solutions. We can provide you the complete service from strategy design to specific execution, target selection, editing scheme customization, editing of large fragment DNA, lentivirus-plasmid library based on CRISPR knockout, genome-wide knockout screening, etc. We also offer TALEN gene knockout, animal model animal gene knockout models and Cas9-Cell Line Gene Knock-Out service (CGKO) for several cell lines and animal models.

We have a strong technical base and a scientific research team that have the experience to develop customized plans for each customer, and use a low-toxicity, high-efficiency and high-specificity RNP delivery mechanism to optimize the rate of success and stability of the experiment.

Capabilities

The following is a breakdown of CRISPR gene editing CRO services:

Service type	Description	Application
Gene editing strategy design	Target selection and editing scheme customization	Basic research, drug screening, disease diagnosis and treatment testing
CRISPR gRNA synthesis service	Provide gRNA synthesis services, including chemical synthesis and in vitro transcription	Gene editing, cell line construction
Construction of CRISPR gRNA library	Construction of CRISPR gRNA library for high-throughput screening	Gene function research, drug target discovery
Cellular gene editing services	Construction of cell lines such as gene knockout, gene knock-in, and point mutation	Biomedical research, drug development
Construction of stable transforming cell lines	Provide stable transformation cell line construction services for multiple cell lines	Biomedicine, gene therapy
CRISPR RNP Delivery Service	Provide CRISPR RNP delivery system for efficient gene editing	Gene therapy, in vivo gene editing
Animal model gene editing	Construction of gene knockout animal models, such as rat and mouse models	Biomedical research, disease models
Microbial genome modification	Genomic modification of microorganisms, such as Escherichia coli, Salmonella, etc.	Agricultural research, industrial applications
CRISPR testing customized services	Provide customized CRISPR testing services, including dual gene and protein testing	Evaluation of gene editing effects, drug research and development
Monoclonal Screening Service	Provide monoclonal screening services to ensure the specificity of gene editing	Cell line construction and gene function study
Gene editing carrier services	Provide gene vector services such as plasmid construction and virus packaging	Gene therapy, drug development

Advantages

Efficient gene editing

Based on various CRISPR/Cas systems, efficient and precise experiments are achieved, with a knockout efficiency of over 98%.

Cost-effectiveness

Outsourcing services can save on investment in equipment, personnel and training, providing affordable options for research institutions and biotechnology companies.

Flexibility and scalability

Outsourcing service providers can quickly adapt to changes in project needs and schedules, supporting researchers to adjust the scale of gene editing projects as needed.

Quality assurance

Outsourcing services ensure that gene editing projects follow the highest standards and provide reliable and repeatable results.

Personalized service

Customize the plan according to customer needs, regularly provide feedback on project progress, and provide detailed delivery reports.

Services Process

We provide one-stop CRISPR-based gene editing services. Our service process is as follows:

Gene editing strategy development

First, it must be discussed with customers on what they want to research and what targets to select and how edit plans should be tailored. This procedure guarantees gene editing is suitable for the client's research objectives.

gRNA design and production

Create and manufacture sgRNA from the customer's gene or editing area. That may include chemical synthesis or in vitro transcription to make gRNA.

Vector construction and delivery vector design

Clone the gRNA to be delivered in the vector, and choose the vector delivery vector (RNP delivery, lentivirus packaging, plasmid transfection).

Transfection of cells, culture of monoclonal cells

Editing vector is introduced into target cells, and the monoclonal cells are screened and cultured. This is the part that makes editing work and cells survive.

Gene editing effect verification

Check the gene editing effect through sequencing, PCR, etc. for exactness and specificity of editing.

Gene editing strategy development

gRNA design and production

Vector construction and delivery vector design

Transfection of cells, culture of monoclonal cells

Gene editing effect verification

FAQs

Here are some frequently asked questions and answers about CRISPR gene editing services:

Q: What is CRISPR gene editing technology?

A: CRISPR gene editing system is an CRISPR/Cas genome editing system. It can precisely insert mutations into DNA sequences to generate gene knockouts, knock-ins or modifications.

Q: What is included in your CRISPR gene editing service?

A: Our CRISPR gene editing services range from Gene editing strategy development, target design, editing scheme design, Large fragment DNA editing, CRISPR knockout lentivirus and plasmid library building, Genome knockout screening, etc.

Q: So how do you make CRISPR editing more effective?

A: Techniques for optimizing CRISPR gene editing are to optimize the crRNA and sgRNA design, delivery mechanisms (including RNP delivery vehicles), and cell transfection techniques.

Q: How do you make the CRISPR editing specific, and avoid off-target effects?

A: We select for a highly specific RNP delivery system and detect the editing effect using two detection of genes and proteins (thereby making sure the editing is highly specific and precise).

Q: And what cell lines/animal models do you offer gene editing of?

A: Our solutions are applicable for various cell lines and animal models like rats, mice, zebrafish etc.

Q: What to do about off-target effects in CRISPR gene editing?

A: The off-target effects could be mitigated by design optimization of crRNA and sgRNA. Also, if an RNP is delivered in an ultra-efficient way, it can minimize off-target effects as well.

Q: How do you verify the results of CRISPR gene editing?

A: We verify all allelic modified cell lines by mRNA or protein levels and provide detailed project reports to ensure the accuracy and reliability of the results.

Q: Are the CRISPR services you provide suitable for all species?

A: Yes, our services cover multiple species, including humans, mice, rats, etc., and we can customize editing plans for specific species according to customer needs.

Q: How to get a quote for CRISPR gene editing services?

A: You can obtain detailed quotation information by filling out the inquiry form or contacting our technical support team directly.

Q: Do the CRISPR services you provide comply with ethical and regulatory requirements?

A: Yes, our CRISPR services strictly follow scientific ethic-related laws and regulations and ensure that all projects comply with regulatory requirements.

Please note that all services are for research use only. Not intended for any clinical use.

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