Strain Engineering for Inulinase

CD Biosynsis' powerful and innovative technology in synthetic biology provides an excellent platform for the production of inulinases and the engineering of microorganisms with more desirable properties. We are committed to helping our customers advance the large-scale synthesis of inulinase in a more efficient and cost-effective manner to meet the growing demand for inulinase in the medical and food industries.

Advantages of Synthetic Biology-Driven Inulinase Production

Inulinase is an inulin catalyzing enzyme belonging to the glycoside hydrolases (GH) family 32, which specifically acts on β-2, 1 linkage of inulin to produce fructose or fructooligosaccharides. Inulinase is produced from a variety of sources, such as animals, plants, and a variety of microorganisms including bacteria, fungi, and yeasts. However, inulinase from animal and plant sources cannot be commercially exploited because of its low yield. Inulinases of microbial origin are ideal for inulinase production due to their ease of handling, culture, and genetic manipulation. However, there are still many challenges in developing a commercial process for the production of inulinase from microorganisms. The most important problems include low yield of bacterial inulinase, poor functional and storage stability, and difficulty in recovery from the reaction mixture. Recent breakthroughs in synthetic biology tools based on nanoparticle immobilization technology have created possibilities for the development of high-quality and highly stable inulinase, which will greatly enhance the production yield and functional properties of inulinase.

Figure 1. Crystal structure of exoinulinase from Aspergillus awamori. (Singh RS, et al., 2020)

What We Provide

CD Biosynsis' advanced synthetic biology platform and extensive expertise allows us to help our customers develop customized and effective strategies to achieve improved production of inulinases.

Deliverables

• Inulinase-producing microorganisms.
• High-quality inulinase products.

How We Can Help

CD Biosynsis' expertise in bacterial synthetic biology and fungal synthetic biology can help our customers solve any problems that may be encountered in the microbial production of inulinase. We are committed to developing effective synthetic biology strategies to improve the stability of inulinase and help our customers achieve large-scale, efficient commercial production.

Engineering of Microbial Chassis for Improved Inulinase Production

Many microbial strains can be developed as high-quality synthetic biology chassis for enhanced inulinase production. We have developed well-established synthetic biology tools and platforms for metabolic engineering of inulinase-producing microorganisms, aiming to achieve large-scale inulinase production in bioreactors. The following microbial strains, including bacteria and fungi, can be used for inulinase production. If you are interested in other strains, please fill out the online inquiry form and tell us more about your project.

Immobilization of Inulinase on Nanoparticles

We have a comprehensive nanotechnology toolbox and a fully integrated platform to help our customers achieve immobilization of inulinases on nanoparticles to increase enzyme thermostability, enable the reuse of enzymes, and reduce costs. Our dedicated scientists can develop customized solutions to help our customers solve problems they may encounter in inulinase production.

Applications of Inulinases

CD Biosynsis leverages our expertise and extensive experience to provide our customers with customized synthetic biology-driven inulinase production solutions to advance the applications of inulinase in the following areas.

• Using inulinase to produce high fructose syrup.
• Using inulinase to produce Inulooligosaccharides.
• Using inulinase to produce ethanol, mannitol, butylene glycol, lactic acid, sorbitol syrup, etc.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for industrial enzyme production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

1. Singh RS, et al. Updates on inulinases: Structural aspects and biotechnological applications. Int J Biol Macromol. 2020, 164:193-210.
2. Singh RS, et al. A panorama of bacterial inulinases: Production, purification, characterization and industrial applications. Int J Biol Macromol. 2017, 96:312-322.
3. Shukla P. Synthetic Biology Perspectives of Microbial Enzymes and Their Innovative Applications. Indian J Microbiol, 2019, 59(4): 401-409.

Please note that all services are for research use only. Not intended for any clinical use.