Strain Engineering for Acrylic Acid

CD Biosynsis has established an integrated synthetic biology platform to help our customers achieve large-scale, low-cost, environmentally friendly, and sustainable production of bio-based acrylic acid (AA). We are committed to developing effective synthetic biology strategies to improve the success rate, competitiveness, and commercial viability of the bio-based routes for AA production.

Advantages of Synthetic Biology-Driven Acrylic Acid Production

Acrylic acid with the molecular formula C₃H₄O₂ is an important C3 platform chemical used as a raw material for a wide range of consumer and industrial products with high added value. In order to reduce the environmental pollution caused by the chemical process of producing AA from nonrenewable petroleum-based feedstocks, continued efforts are being made to develop microbial-based methods that have the advantages of simple process steps, availability of a wide range of renewable feedstocks and low cost. However, the challenges that arise in AA bioproduction, such as low yield, often cannot be addressed by conventional methods of overexpressing or knocking out single or few target genes in acrylic acid-producing microorganisms. The rapid development of synthetic biology has made it possible to construct more efficient microbial strains and cell factories to improve the production of AA.

Figure 1. The biosynthetic pathway designed for the production of acrylic acid, and key metabolic engineering strategies. (Ko Y S, et al., 2020)

What We Provide

Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of acrylic acid.

Deliverables

• Acrylic acid-producing microorganisms.
• High-quality acrylic acid products.

How We Can Help

CD Biosynsis has a team of scientists working to accelerate the transition from fossil-based AA production to synthetic biology-driven microbial production. We can provide our customers with innovative and sustainable approaches for production of AA at high yield and high purity.

Microbial Chassis Development for AA Production

We have established a microbial strain collection with the potential to be developed as synthetic biology chassis by screening, identifying, and characterizing acrylic acid-producing strains and determining their tolerance to AA. We can help engineer microbial cells to improve AA production or construct more efficient strains for heterologous production of AA. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.

Heterologous Production of AA

We can develop synthetic biology tools and metabolic engineering strategies to design novel biosynthetic pathways for heterologous production of AA with or without 3-hydroxypropionic acid (3-HP) as an intermediate in microbial strains such as E. coli. Effective strategies will be developed to decrease the formation of by-products and increase the metabolic flux toward the biosynthetic pathway to improve the yield of AA. Metabolic pathways can be further optimized by introducing efficient enzymes screened out from microbial sources. Our scientists can help our customers select the most attractive routes for heterologous production of AA and construct high-efficiency cell factories.

Applications of Acrylic Acid

CD Biosynsis leverages our multidisciplinary capabilities and advanced synthetic biology platform to support the wide applications of AA in various industries.

• Used as a chemical intermediate in the production of ethyl acrylate, methyl acrylate, and other specialty acrylates.
• Used in the preparation of polymers, such as superabsorbent polymers (SAPs), emulsion polymers, and detergent polymers.
• Used in the food packaging industry.
• Used in the production of a wide variety of products, such as acrylic paints, plastics, adhesives, binders for paper, leather and textiles, decorative, masonry and industrial coatings, elastomers, polishes, synthetic rubber, disposable diapers, water treatment chemicals, and detergents.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

1. Ko Y S, et al. A novel biosynthetic pathway for the production of acrylic acid through β-alanine route in Escherichia coli. ACS Synthetic Biology, 2020, 9(5): 1150-1159.
2. Rodrigues J L. Heterologous production of acrylic acid: current challenges and perspectives. SynBio, 2022, 1(1): 3-32.

Please note that all services are for research use only. Not intended for any clinical use.