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Trusted by Leading Research & Pharma Institutions

Protein Expression in E. coli

High-yield E. coli protein expression service with rapid turnaround, comprehensive optimization strategies, and flexible scale options for your research needs.

High Success Rate
Fast Turnaround
Flexible Scale
Learn More

Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Roche
Stanford
Novartis

Why Choose Us

Comprehensive expression optimization
Multiple fusion tag options
Scalable from mg to multi-gram
Expert consultation included

T7 Expression System

Industry-standard BL21(DE3) strains

Quality Guaranteed

MS verification & endotoxin control

Flexible Options

His, SUMO, GST, MBP tags

Success Rate
High
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Why Choose Our E. coli Expression Service?

Advanced expression technologies combined with optimized protocols deliver superior results for your recombinant protein projects.

Rapid Turnaround

Optimized protocols enable fast delivery while maintaining highest quality standards for your protein expression projects.

  • Streamlined expression workflow
  • High-throughput screening
  • Flexible timeline options

High Success Rate

Comprehensive condition optimization ensures successful expression even for challenging targets including toxic proteins.

  • Multiple host strain options
  • Solubility enhancement strategies
  • Expert troubleshooting support

Comprehensive Tags

His, SUMO, Trx, GST, MBP, and NusA to maximize expression.

Scalable Production

From mg to multi-gram scale with seamless scale-up.

Flexible Options

Custom vector construction and expression strategies.

Quality Guaranteed

MS verification and endotoxin control on every batch.

Advanced Expression Technologies

Cutting-edge technologies combined with proven optimization strategies maximize your protein expression success.

Expression System

T7 Expression System

Industry-standard T7 promoter-based expression system for high-level protein production in BL21(DE3) derivative strains.

  • T7 RNA polymerase-driven transcription
  • IPTG-inducible expression control
  • Low basal expression background
Solubility Enhancement

Fusion Tag Library

Comprehensive collection of solubility-enhancing tags to improve protein folding and simplify purification workflows.

  • His-tag for IMAC purification
  • SUMO, Trx, GST for solubility
  • MBP, NusA for difficult proteins
Host Engineering

Strain Optimization

Extensive collection of engineered E. coli strains for challenging expression targets and specific protein requirements.

  • BL21(DE3) for standard expression
  • Rosetta strains for codon bias
  • SHuffle for disulfide bonds
Sequence Optimization

Codon Optimization

AI-powered codon optimization algorithms to enhance translation efficiency and maximize protein yield.

  • E. coli codon usage adaptation
  • mRNA secondary structure optimization
  • RBS sequence optimization
Protein Refolding

Inclusion Body Refolding

Proprietary refolding protocols for recovering bioactive proteins from inclusion bodies with high recovery rates.

  • Multiple refolding buffer conditions
  • Gradient dilution methods
  • Redox optimization for disulfide
Screening Platform

High-Throughput Screening

Automated screening of multiple expression conditions to identify optimal protocols for your specific protein.

  • Multiple temperatures tested
  • Various inducer concentrations
  • Different host strains comparison

Service Specifications

Comprehensive specifications for your protein expression projects.

ParameterSpecifications
Expression SystemE. coli BL21(DE3) and derivative strains
Vector OptionspET series (pET-21, pET-28, pET-32), pGEX, pCold, pBAD
Fusion TagsHis, SUMO, Trx, GST, MBP, NusA, thioredoxin, optional tag removal
Protein Size Range5 kDa - 150 kDa
Expression YieldsHigh yield in optimized conditions
Purity LevelsStandard (SDS-PAGE), high purity, ultrapure grade available
Endotoxin LevelsStandard and low endotoxin options available
Delivery FormatsLiquid (buffered), lyophilized, conjugated
Scale OptionsMilligram to multi-gram scale; fermentation scale available
QC Verified
Purity Confirmation
Endotoxin
Control Options
MS Verified
Sequence Confirmation
CoA Included
Full Documentation

Our Streamlined Workflow

Efficient process from sequence to purified protein delivery.

1

Sequence Analysis & Gene Optimization

Our team analyzes your protein sequence for codon usage, mRNA secondary structure, and potential expression challenges. AI-powered optimization ensures maximum translation efficiency.

Week 1
2

Vector Construction & Cloning

Gene is synthesized and cloned into your chosen expression vector with optimal fusion tag. Rigorous sequencing verification ensures sequence accuracy.

Week 2
3

Expression Optimization & Screening

We screen multiple expression conditions including different host strains, temperatures, and induction parameters to identify optimal production conditions.

Week 2-3
4

Scale-Up & Purification

Using optimized conditions, we scale up production and purify your protein using appropriate chromatography methods including IMAC, ion exchange, and SEC.

Week 3-4
✓

Quality Control & Delivery

Comprehensive QC analysis including SDS-PAGE, endotoxin testing, and optional activity assays. Your protein is delivered with Certificate of Analysis and technical report.

Delivery
Applications

Diverse Applications Across Research

Our E. coli expression services support research and development in multiple fields.

Structural Biology Applications

High-purity E. coli-expressed proteins ideal for X-ray crystallography, NMR spectroscopy, and cryo-EM studies.

  • Crystallography-grade purity by SEC-HPLC
  • Isotope-labeled proteins for NMR
  • Consistent batch-to-batch quality
  • Low endotoxin for structural studies
High
Crystallography Success

Biochemistry & Enzymology

Enzymes and binding proteins with verified biological activity for biochemical assays and kinetic studies.

  • Enzyme kinetics characterization
  • Binding assays and Kd determination
  • Activity verification included
  • Multiple buffer formulations
Verified
Bioactivity Assays

Drug Discovery & Screening

High-quality proteins for HTS, hit validation, and lead optimization in drug discovery programs.

  • HTS-compatible formats
  • Bulk production capabilities
  • GMP-grade available
  • Flexible packaging options
Scalable
Drug Discovery Support

Antibody Development

Antigen proteins for immunization, hybridoma development, and antibody validation studies.

  • Conjugation-ready antigens
  • Multiple species variants
  • Carrier protein conjugation available
  • Sequential immunization support
Pure
Antigen Quality

What Our Customers Say

Trusted by researchers worldwide for reliable protein expression services.

"The expression optimization service helped us obtain soluble protein from a difficult target. The team's expertise in E. coli expression was evident throughout the project."

RC

Research Director

Biotechnology Company

"Fast turnaround and excellent communication. We received our first batch of pure protein quickly. The quality exceeded expectations for crystallography studies."

JW

Principal Investigator

Academic Research Institution

"We've scaled up production with this service for drug discovery. The consistency between batches has been remarkable for our HTS assays."

SM

Senior Scientist

Pharmaceutical Company

Scientific Literature

Backed by peer-reviewed research and continuously improved based on latest scientific advances.

85
Citations

Strategies for efficient production of recombinant proteins in E. coli: alleviating host burden and enhancing protein activity

Zhang W, et al. Microbial Cell Factories (2022)

View DOI
20
Citations

Stepwise optimization of recombinant protein production in E. coli utilizing computational and experimental approaches

Packiam KAR, et al. Applied Microbiology and Biotechnology (2020)

View DOI
42
Citations

Efficient Production of Fc Fusion Proteins in the Cytoplasm of E. coli: Dissecting and Mitigating Redox Heterogeneity

Tungekar AA, et al. International Journal of Molecular Sciences (2022)

View DOI
15
Citations

Genome-scale metabolic model-based engineering of E. coli enhances recombinant scFv production

Behravan A, et al. Biotechnology Letters (2022)

View DOI
156
Citations

New tools for recombinant protein production in E. coli: A 5-year update

Rosano GL, et al. Protein Expression and Purification (2019)

View DOI

Frequently Asked Questions

Find answers to common questions about our E. coli expression service.

What is the typical turnaround time?
Standard service takes a few weeks from sequence submission to protein delivery. This includes gene synthesis, cloning, expression optimization, scale-up, and purification. Rush options are available for time-sensitive projects.
What fusion tags do you offer?
We offer His-tag (standard for IMAC purification), SUMO, Trx (thioredoxin), GST, MBP (maltose binding protein), and NusA tags. Each tag has unique benefits for solubility enhancement. Tag removal using TEV or SUMO protease is available upon request.
Can you express difficult proteins like membrane proteins or toxic proteins?
Yes. We have specialized strains for challenging targets including SHuffle (for disulfide-bonded proteins), Origami (for oxidative folding), Rosetta (for rare codon-containing genes), and tunable expression systems (for toxic proteins). High-throughput screening helps identify optimal conditions.
What purity levels can you achieve?
Standard purity is provided by SDS-PAGE analysis. High purity option and ultrapure grade are available with additional chromatography steps. We also offer endotoxin removal options for cell-based assays.
Do you offer inclusion body refolding services?
Yes. For proteins that express as inclusion bodies, we offer comprehensive refolding services. Our optimized refolding protocols include multiple buffer conditions screened to maximize recovery of bioactive protein. Redox optimization is available for disulfide-bonded proteins.
What scale of production is available?
We offer scales from milligram (research grade) to multi-gram and larger (production grade). Standard service includes purified protein. Larger scales are available with fermentation options. Custom scale-up consultations are provided.
What quality control is included?
Every protein comes with comprehensive QC data including SDS-PAGE analysis, endotoxin testing, and sequence verification by mass spectrometry. Certificate of Analysis (CoA) documents all quality specifications. Optional activity assays and SEC-HPLC purity analysis are available.

Ready to Start Your Project?

Get a customized quote for your Protein Expression in E. coli project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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