Rational Protein Design

CD Biosynsis provides professional rational protein design services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Introduction to Rational Protein Design

Rational protein design is a great challenge to our understanding of protein structure and physical chemistry. It has been widely used in numerous fields, including synthetic biology. Rational design is the most significant approach of protein engineering based on site-directed mutagenesis. The strategy is based on the relating structure to function, frequently via molecular modeling techniques. Rational design requires the knowledge of the amino acid sequence, 3D structure and function of the target protein. This method provides controllable amino acid sequence changes such as insertion, deletion, substitution, exchange of secondary structural elements, exchange of the entire domain, or fusion of enzymes to create new proteins.

Figure 1. Designed enzyme therapeutics. (Wilson C J., 2015)

Service Offering

Based on the understanding of the target protein’s structure and the correlated properties, CD Biosynsis’ professional scientific team can provide our customers with a complete development cycle of rational design.

Figure 2. Workflow of our rational protein design services.

Protein Structure Determination

We provide high-quality one-stop protein structure determination services, including XRC, NMR, Cryo-EM, and structure prediction services, in order to help our customers better understand the function of interested proteins. This also has a guiding significance for computer-aided design.

Computer-Aided Design

Computational-aided design (CAD) is a practical tool to help identify amino acid sequences that can improve protein stability, modify binding, increase protein solubility, or affect protein function to meet the requirements of researchers. We combine structural biology, bioinformatics and computer science to provide our customers with high-quality CAD services.

Site-Directed Mutagenesis

Site-directed mutagenesis is based on the principle of introducing real amino acids into target genes. It is one of the most widely used strategies for the molecular modification of proteins. With years of experience in the field of molecular biology, we can provide fast and economically site-directed mutagenesis services to meet customers’ needs.

Cell-Based Protein Expression

Once the site-directed mutation is successful, the plasmid with the mutated gene is transformed into the expression system to express the target protein. In this process, an exciting progress is the ability to integrate unnatural amino acids(uAAs) (i.e. not found in standard amino acids) into proteins, thereby modifying proteins with higher selectivity and efficiency. As a leader in providing recombinant protein expression services, we have more than 10 years of extensive experience in custom protein expression.

Protein Characterization

After expression and purification, we need to characterize the new protein to make sure the desired property is achieved. If not, the information obtained in the evaluation is used in the second round of modeling, mutagenesis, and characterization. With world-class analytical techniques, we are proud to provide a wide range of characterization services to ensure the successful construction of the desired property.

CD Biosynsis has been involved in the field of protein design and engineering for many years. We are fully committed to working with you to facilitate the successful completion of the projects. If you are interested in our services, please contact us for more details.

References

1. Wilson C J. Rational protein design: developing next‐generation biological therapeutics and nanobiotechnological tools. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2015, 7(3): 330-341.
2. Hellinga H W. Rational protein design: combining theory and experiment. Proceedings of the National Academy of Sciences, 1997, 94(19): 10015-10017.

Please note that all services are for research use only. Not intended for any clinical use.