Strain and cell development services

CD Biosynsis powers revolutionary research and development via our sophisticated Strain and Cell Development Services which adapt to the changing needs of biotechnology fields and synthetic biology applications. Our team combines genetic engineering mastery with advanced technological platforms to develop customized solutions that simplify the processes of developing and verifying efficient microbial strains and engineered cell lines. Our use of precise delivery systems that reduce toxicity leads to better experimental outcomes and stable engineered strains and cells over time. Our comprehensive support system accelerates all development stages from strategic planning to scalable execution for goals related to metabolic pathway optimization, recombinant protein production, or functional genomics.

Get a Quote

Overview Services Offered Services Workflow Applications Case studies Customer Reviews FAQs Contact Us Related Services Related Resources

What is Strain and cell development services?

Strain and Cell Development Services refer to specialized scientific solutions focused on designing, engineering, optimizing, and validating microbial strains (e.g., bacteria, yeast) or cell lines (e.g., mammalian, plant, or insect cells) for specific research, industrial, or therapeutic applications. These services combine advanced genetic engineering, synthetic biology, and molecular biology techniques to create tailored biological systems with enhanced performance, productivity, or functionality.

Types of DNA and RNA Synthesis Services

By Technology By Species

By Technology

Cell Line Development

Stable Cell Line Development

Includes CHO cell engineering service for recombinant protein production and industrial applications.

Inducible Cell Line Development

Enables temporal control of gene expression using chemical or environmental triggers.

Reporter Cell Line Development

Integrates fluorescent or luminescent markers for real-time monitoring of gene activity.

Genetic Engineering

Gene Insertion/ Deletion/ Silencing (RNAi)/ Overexpression

Precise modification of target genes for functional studies, pathway optimization, and therapeutic development.

CRISPR-Cas9 Genome Editing

High-throughput, site-specific editing in bacteria (e.g., E. coli), yeast, and mammalian cells.

Inducible Expression Systems

Promoter Engineering

Design of customizable promoters for tunable gene expression.

Inducer Optimization

Tailored chemical or environmental cues to maximize expression efficiency.

Regulatory Element Modification

Fine-tuning of enhancers, repressors, and other cis-acting elements.

Metabolic Engineering

Optimization of biosynthetic pathways in bacteria (e.g., E. coli) and plants for chemical production.

By Species

Bacteria

E coli Metabolic Engineering

Metabolic engineering for biofuel/chemical synthesis.

E coli Gene Knockout

Gene knockout and CRISPR-Cas9 editing for strain optimization.

Yeast

Saccharomyces cerevisiae Genetic Engineering

Genetic engineering for bioethanol production and drug discovery.

Pichia pastoris Glycoengineering

Glycoengineering to improve therapeutic protein quality.

Yeast Surface Engineering

Cell surface engineering for biocatalysis and strain improvement.

Algae

Algae Photosynthetic Efficiency Improvement

Enhanced biomass production for biofuels and carbon sequestration.

Plants

Plants Metabolic Pathway Engineering

Crop improvement and biosynthesis of pharmaceuticals (e.g., antibodies).

Molecular Farming

Production of recombinant proteins in plant tissues (e.g., seeds, leaves).

Animal Cells

CHO Cells

Genetic engineering and recombinant protein expression for biopharmaceuticals.

HEK Cells

Genetic modification and viral vector production for gene therapy.

Workflow for Strain and Cell Development Services

Demand Communication and Project Formulation

Contract Agreement

Strain/Cell Development Execution

Results Delivery and Post-Project Support

Needs Assessment: CD Biosynsis clients work with technical consultants to establish project goals by setting desired traits such as enhanced protein expression and metabolite production while choosing host organisms including bacterial, yeast, and mammalian cells and determining applications like bioproduction and disease modeling.

Strategic Planning: A specialized workflow comes from the technical team which chooses suitable host strains along with genetic tools like CRISPR and TALENs to implement editing strategies. The project plan with timelines and deliverables requires client approval before moving forward.

Upon finalizing the project plan, a service contract is signed to confirm scope, costs, timelines, and intellectual property terms.

The experimental phase is executed through a systematic approach:

a. Host Strain Selection and Genetic Design

Host Selection: Optimal microbial strains or cell lines are chosen based on compatibility with target modifications and scalability.

Construct Design: Custom genetic constructs (e.g., vectors, gRNAs, donor templates) are designed for precise editing. Regulatory elements (promoters, selection markers) are incorporated to ensure efficient expression and screening.

b. Genetic Modification

Editing Delivery: Editing tools (e.g., CRISPR/Cas9 systems) are introduced into the host via transformation (bacteria/yeast) or transfection (mammalian cells), using methods like electroporation or viral vectors.

Modification Types: Services include knockouts, knock-ins, point mutations, or pathway engineering, depending on client requirements.

c. Screening and Validation

Primary Screening: Edited clones are selected using antibiotic resistance, fluorescence markers, or auxotrophic selection.

Genotypic Validation: PCR, sequencing, and Southern blotting confirm genetic accuracy and rule out off-target effects.

Phenotypic Testing: Functional assays (e.g., enzyme activity, growth kinetics, metabolite profiling) validate desired traits.

d. Process Optimization and Scale-Up

Culture Optimization: Growth conditions (media, pH, temperature) are refined to maximize productivity and stability.

Scale-Up: Validated strains/cells are cultured in bioreactors or large-scale fermenters to generate bulk quantities for industrial or clinical use.

Final Deliverables: Clients receive genetically modified strains/cell lines, accompanied by a comprehensive report detailing editing efficiency, phenotypic data, scalability metrics, and quality control analyses (e.g., genetic stability, purity).

Technical Support: CD Biosynsis offers post-delivery assistance for troubleshooting, further optimization, or downstream applications (e.g., fermentation protocols, cryopreservation guidance).

Applications of Strain and Cell Development Services

Bioproduction and Industrial Biotechnology

High-value compound production: Through genetic engineering scientists can direct E. coli and yeast to produce insulin and antibiotics for pharmaceutical use while simultaneously producing biofuels and enzymes for industrial applications.
Metabolic pathway optimization: Engineered microbial strains should have their metabolic flux pathways optimized to boost the production yields of valuable substances including amino acids and bioplastics.
Scalable protein expression: Develop CHO and HEK293 cell lines specifically engineered to yield high amounts of monoclonal antibodies and recombinant proteins.

Biomedical Research and Therapeutic Development

Cell therapy engineering: Engineer CAR-T and NK cells with synthetic receptors for precise cancer immunotherapy delivery.
Viral vector production: HEK293T packaging cell lines are specifically engineered to generate lentiviral and AAV vectors which are used in gene therapy applications.
Disease modeling: Researchers use CRISPR genetic modification techniques to build cell lines which allow scientists to study disease development and test drug reactions.

Synthetic Biology and Metabolic Engineering

Biosensor design: Develop microbial genetic circuits to work as detectors for environmental toxins and biological markers.
Artificial metabolic pathways: Scientific research teams use synthetic gene clusters within microbes to develop new biochemical products and essential compounds for pharmaceutical applications.
Codon optimization: Genetic code modification allows for improved protein production from foreign DNA across bacterial, yeast and mammalian expression platforms.

Agricultural and Environmental Biotechnology

Biofertilizer development: Research into genetically modified Pseudomonas bacteria which enhance plant growth leads to higher crop yields.
Bioremediation strains: Scientists genetically modify microorganisms to break down environmental pollutants like oil and pesticides.
Algal biotechnology: Scientific research groups must develop specialized microalgae strains to enhance carbon capturing abilities and increase biofuel production.

Diagnostics and Vaccine Development

Diagnostic reagent production: Engineer cell lines that generate viral antigens along with the SARS-CoV-2 spike protein to be used in antibody testing.
Vaccine substrates: Yeast cells or insect cells serve as essential substrates for producing subunit vaccines and virus-like particles (VLPs).
Antibiotic susceptibility testing: Engineer bacterial strains that respond to antimicrobial treatments as reporting systems.

Case studies of Strain and Cell Development Services

Gene fragment knockout-Gene A

Fig1: PCR-agarose gel electropherogram (partial results)

Fig2: Comparison results of some positive samples after sequencing

What Our Clients Say

We partnered with CD Biosynsis to overcome difficulties in microbial strain optimization for enhanced protein production. Our protein expression levels rose by 35% through the integration of advanced strain development with AI-based metabolic pathway analysis. The team's specialized knowledge in genome editing and fermentation optimization greatly shortened our research and development schedule. Quality standards and innovative practices from their team played an essential role in improving our bioproduction capabilities.

Dr. A. Thompson, Head of Bioprocess Development

CD Biosynthesis delivered outstanding assistance for creating a high-performance cell line to meet our antibody production requirements. CD Biosynsis's proprietary cell engineering platform and strict quality control processes produced a cell line with 98% viability while cutting production costs by 50%. The team displayed excellent technical expertise and responsiveness which streamlined and optimized the process. Anyone seeking dependable cell development solutions should consider using their services.

Ms. R. Patel, Senior Scientist, Biopharmaceuticals

Our collaboration with CD Biosynsis helped us overcome scaling obstacles in our microbial fermentation process. The company's custom strain development strategy and advanced analytics resulted in a 40% increase in product yield and a 30% reduction in fermentation time. Their team has become our trusted partner because of their commitment to custom solutions and meeting tight deadlines.

Mr. J. Kim, Director of Fermentation Sciences

Faced with low productivity levels in our yeast-based biofuel production lines we sought out the strain development expertise of CD Biosynsis. Their advanced metabolic engineering techniques alongside strong screening protocols developed a strain that achieved a 45% increase in ethanol production. The team completed their work within a narrow time limit which was impressive and their solutions greatly improved our production efficiency.

Mr. T. Nguyen, Lead Scientist, Biofuels Division

FAQs about Gene Editing Services

Still have questions?

Which strain development services does your company provide?

Our strain development services encompass microbial strain optimization and metabolic engineering as well as genome editing and high-throughput screening specifically for bioproduction biofuels and pharmaceutical applications.

Is it possible to create tailored cell lines that meet specific application requirements?

We develop custom cell lines tailored to your needs which include CHO cells for antibody production, HEK cells for protein expression and microbial strains for industrial applications.

Which technologies are employed in your strain and cell line development processes?

Our development of optimal strains and cell lines relies on advanced CRISPR-Cas9 genome editing technologies alongside AI-driven metabolic pathway analysis and high-throughput screening platforms.

The development time for an optimal strain or cell line depends on the project complexity and generally lasts between 4 to 12 weeks.

Our project timeline depends on complexity and generally falls between 4 and 12 weeks. High-throughput screening and optimization processes aim to deliver faster results while maintaining high quality standards.

Are your services available for scaling up strains or cell lines to meet industrial production needs?

Our services cover the complete process from strain and cell line development through fermentation optimization and scale-up to facilitate smooth progress from laboratory to industrial production.

Which quality control protocols are implemented during the development of strains and cell lines?

Our quality control protocols combine genomic sequencing with phenotypic characterization and performance validation to maintain reliable and consistent developed strains and cell lines.

Is it possible to enhance productivity through optimization of existing strains or cell lines?

Absolutely. Through advanced metabolic engineering and optimization techniques we analyze and re-engineer existing strains or cell lines to boost productivity while improving yield and lowering production expenses.

Which industries receive strain and cell development solutions from your company?

We offer services to multiple sectors such as biopharmaceuticals, biofuels production, agriculture, food and beverage industries and industrial biotechnology.

Is regulatory compliance support available for your strain and cell line development process?

Our team provides both guidance and documentation that helps developed strains and cell lines satisfy regulatory requirements for pharmaceuticals and biologics as well as other regulated industries.

What measures do you take to protect the confidentiality of our project details?

Our organization places client confidentiality as our primary concern and implements rigorous data protection measures. We protect all project information along with intellectual property through the highest standards of security and confidentiality.