CD Biosynsis offers our customers high-efficiency DNA and RNA synthesis services that deliver accurate and complete results. Our team combines deep expertise with cutting-edge technology platforms to develop tailored solutions that address various requirements including gene design and functional validation. The implementation of highly efficient and specific delivery systems that minimize toxicity leads to enhanced success rates and improved experimental stability. Our services provide complete process support from designing gene editing strategies to CRISPR gRNA synthesis, constructing gene libraries and transforming cell lines enabling customers to accelerate their research progress and achieve innovative objectives. By selecting CD Biosynsis you gain access to top-notch services that are both efficient and budget-friendly which will propel your research forward through breakthrough achievements.
Get a QuoteDNA and RNA synthesis services represent professional biotechnology offerings designed to deliver researchers with tailor-made high-quality DNA and RNA molecules. Many research fields including gene function analysis together with novel therapeutic approaches and gene editing utilize these DNA and RNA synthesis services. DNA synthesis describes the process of building specific DNA sequences in external environments using chemical or enzymatic techniques. Contemporary DNA synthesis technology enables efficient production of DNA fragments from short sequences to ultra-long strands exceeding 10Kb. The process of RNA synthesis involves the production of distinct RNA molecules through chemical or enzymatic methods in an extracellular environment. RNA synthesis services offer a comprehensive range which covers standard RNA synthesis methods alongside advanced techniques for modified RNA synthesis and chimeric DNA/RNA structures as well as siRNA and miRNA production. Modern molecular biology research relies heavily on DNA and RNA synthesis services which enable scientists to conduct gene function analysis and develop drugs while advancing gene editing techniques.
Custom DNA synthesis
This service creates DNA sequences through design and synthesis that meet particular customer specifications. This service enables researchers to produce DNA sequences with exact nucleotide sequences and alterations that serve purposes including gene cloning and DNA diagnostics.
This method uses microarray technology to produce multiple DNA sequences simultaneously. The rapid production of DNA libraries at low cost through this high-throughput method supports genome-wide association studies and synthetic genomics as well as gene expression profiling.
This approach targets the creation of specific gene fragments instead of whole genes. The purpose of this service is to generate specific gene regions that are essential for research applications like mutagenesis studies, protein domain analysis, and creating chimeric genes.
This service provides expertise in the creation of single-stranded DNA molecules. The unique properties of ssDNA enable its use as molecular diagnostic probes and DNA sequencing templates along with its role as a component in specific enzymatic reactions.
This synthesis technique produces DNA sequences that extend from several hundred base pairs up to thousands of base pairs in length. The synthesis of large DNA constructs which is crucial for genome editing and gene therapy vector development along with the assembly of complex genetic circuits is performed by this service.
This service synthesizes DNA sequences that possess specific chemical modifications. These modifications may involve fluorescent labels, biotin tags, methylations or additional functional groups and they serve functions such as DNA tracking detection and gene expression regulation.
This process requires designing and producing synthetic DNA collections that contain entirely predetermined sequences. Researchers apply these libraries to direct evolution processes, synthetic genomics research and identifying new biological functions.
The construction of DNA libraries encoding antibody variable regions is the main focus of antibody DNA libraries. The antibody DNA libraries facilitate antibody discovery and engineering processes which produce highly specific and powerful antibodies for use in both diagnostic and therapeutic applications.
The custom RNA oligos synthesis process operates like custom DNA oligos synthesis but targets RNA sequences. The process enables researchers to create specific RNA oligonucleotides with tailored sequences and modifications for multiple RNA research applications.
The process creates small interfering RNAs that contain precise sequences which target designated genes. RNA interference (RNAi) experiments utilize siRNAs to silence genes which allows researchers to study gene functions.
This service specializes in creating microRNAs (miRNAs) which serve as small non-coding RNAs that help control gene expression. Scientists employ custom miRNA synthesis to investigate miRNA functions while simultaneously validating miRNA targets and advancing miRNA-based therapeutic development.
This service produces agomirs and antagomirs which serve as chemically modified miRNAs and miRNA inhibitors respectively. Agomirs serve to increase miRNA activity while antagomirs function to block miRNA activity enabling detailed research into miRNA-related gene control mechanisms.
The synthesis service can create RNA strands that span several hundred to thousands of nucleotides in length. Applications requiring mRNA vaccines production, gene expression vectors and long non-coding RNA research depend on this service.
The service provides RNA sequences manufactured with specific chemical modifications to satisfy exact experimental needs. RNA sequences can be customized with fluorescent labels or biotin tags along with other functional groups to enable diverse applications including tracking RNA molecules and detecting or regulating their activity.
The sgRNA Design Service focuses exclusively on creating sgRNAs for CRISPR-Cas9 genome editing applications. The CRISPR-Cas9 system depends on sgRNAs to accurately guide the nuclease to designated genomic locations for precise genetic modifications.
The process includes synthesizing sgRNAs which have been designed for genome editing using CRISPR-Cas9. Our service delivers high-quality sgRNAs which enable efficient and specific applications in gene editing.
The service offers custom-designed sgRNA libraries for comprehensive genome-wide studies and specific gene editing experiments. Researchers use these libraries to conduct high-throughput screening that reveals genes participating in specific biological processes or phenotypes.
This synthesis process produces oligonucleotide probes that are specifically created for diagnostic applications. Diagnostic probes can bind to specific DNA or RNA sequences which allow for the recognition of disease-related genetic markers.
This service focuses exclusively on the production of oligonucleotides needed for next-generation sequencing technologies. In NGS workflows, these oligos function as primers or adapters that enable DNA or RNA sample sequencing.
The process produces oligonucleotides with precise chemical alterations according to experimental needs. Oligonucleotides can be modified through fluorescent labels, biotin tags, or different functional groups to enable nucleic acid labeling and detection as well as purification processes.
The process involves creating antisense oligonucleotides which match specific mRNA sequences. By binding to specific target mRNAs these oligos disrupt gene expression which helps in conducting gene silencing research and developing new treatments.
The project specializes in creating cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs). The immune system can be activated through CpG ODNs which have applications in immunotherapy treatments and vaccine design.
This process produces libraries composed of specially designed oligonucleotides that feature multiple sequences. Researchers utilize these libraries for multiple scientific applications which include genetic interaction screening, directed evolution experiments and synthetic genomics studies.
The process generates DNA sequence libraries with mutations positioned at specific gene sites. This technique enables exact control of gene function while serving as a fundamental tool in protein engineering and functional genomics research.
Creates DNA sequence libraries where random point mutations are spread across particular gene regions. The approach facilitates systematic investigation into how various mutations affect both gene function and protein activity.
Generates DNA sequence libraries featuring random mutations at various positions throughout a gene. High-throughput screening benefits from this library type to discover novel variants that exhibit desired phenotypes and functions.
The Degenerated Mutagenesis Libraries Service constructs libraries that contain degenerate nucleotide sequences with multiple nucleotide options at specific locations. Researchers can investigate numerous potential amino acid sequences encoded by specific DNA regions through this process.
The construction of human genomic DNA libraries incorporates specific gene knockouts for research purposes. Functional genomics research depends on this service because it enables scientists to study how single genes affect human development processes and diseases.
The service focuses on building CRISPR-based gene knockout libraries specifically for livestock and poultry genomes. Researchers can explore gene functions in key agricultural species through this method which supports advancements in animal breeding and disease resistance.
This service requires the creation of genes and gene fragments from basic elements. Researchers utilize this service in different scientific applications including gene cloning experiments and recombinant protein production along with gene expression analysis.
This service creates cDNA libraries by processing mRNA samples. cDNA libraries contain expressed genes from specific cell types or tissues and researchers use them for discovering new genes as well as profiling expression and cloning targeted genes.
The Gateway cloning technology enables the assembly and cloning of extensive DNA fragments as well as entire genomes. Researchers can build complex genetic constructs through this method because it achieves efficient and precise DNA assembly.
Golden Gate assembly method expertise allows for accurate and efficient integration of multiple DNA fragments into a single assembly. Researchers utilize this service to build synthetic genomes alongside metabolic pathways and genetic circuits.
This service can produce small genomes which usually fall between several kilobases and tens of kilobases in size. This service examines minimal genome requirements for life and develops synthetic organisms and novel biological systems.
The synthesis of adenovirus genomes serves multiple applications including gene therapy treatments, vaccine production and cancer treatment through oncolytic virotherapy. Adenoviruses function as versatile viral vectors because they can transduce numerous cell types with high efficiency.
The service creates antibody genes which produce specific antibodies with targeted properties. The service supports antibody engineering operations such as monoclonal antibody production and the creation of antibody-drug conjugates and bispecific antibodies.
Strict nucleic acid quantification and purity testing
Concentration and purity assessment: Nucleic acid purity concerning protein and organic solvent contamination is confirmed through UV-Vis spectrophotometry by analyzing OD260/280 and OD260/230 ratios.
Integrity verification: The RNA integrity coefficient (RIN value) results from analysis by capillary electrophoresis (CE) or Agilent Bioanalyzer. High-sensitivity experiments including RNA-Seq can use samples with RIN values of 8 or higher to prevent 3'-end bias.
Internationally certified quality management system
ISO standard compliance: Quality control requirements of ISO 20688-2:2024 mandate the assessment of purity along with cloning accuracy and sequence accuracy for synthetic gene fragments and genomes including residual impurity testing.
Customized services and response to customer needs
Flexible specification adaptation: We offer customized synthesis of single-stranded and double-stranded RNA together with modification tags like fluorescence or biotin and siRNA/miRNA for basic research through preclinical development stages.
Rapid response mechanism: Provide technical consultation feedback within 48 hours while adjusting extraction methods or library construction strategies to align with project requirements from customers.
Confidentiality and compliance
Ensure intellectual property protection through signing an NDA agreement.
Basic research and genetic engineering
Biomedical development
Diagnosis and testing technology
Synthetic biology applications
Gene therapy and personalized medicine
Synthesis of 30-base all-thioate DNA
Sequence: DNA 30 base fully thioate
Target molecular weight: 9670
"To solve the problem of GC content deviation in our large-scale siRNA library synthesis, CD Biosynsis designed a dynamic annealing temperature adjustment system. Its chip synthesis platform equipped with triple quality control functions has increased the success rate of oligonucleotide synthesis with complex secondary structures to 98.7%, and shortened the single batch delivery cycle by 40%. This technological innovation directly supports the upgrade of our drug target screening pipeline."
Dr. L. Hernandez, Chief Scientist, Nucleic Acid Therapy
"When developing antiviral transgenic crops, more than 200 guide RNAs are needed for multi-target screening. CD Biosynsis not only achieves 96-hour emergency delivery, but its original tRNA-mediated CRISPR delivery system stabilizes editing efficiency to 92%±3% in monocotyledons. This is breakthrough data that we have never obtained from other suppliers."
Prof. K. Yamamoto, Program Leader of Molecular Agronomy
"For probe design for new zoonotic strains, CD Biosynsis provides ultra-long strand RNA synthesis services (>15kb) to demonstrate excellent integrity. After NGS verification, the consistency of the viral genome fragments synthesized using phosphosulfuryl bond enhancement technology reached 99.4% among three consecutive batches, successfully helping us establish a mutation monitoring system six weeks in advance."
Dr. James Clark, Senior Researcher, Pathogen Evolution Research Group
"When developing personalized TCR-T therapies, CD Biosynsis's methylation sensitive primer synthesis technology greatly improves the identification of mutation sites. Its DNA synthesis service that integrates single-molecule barcode tracking reduces the detection limit for rare clones to 0.01% VAF. This accuracy is a key element in gaining FDA approval for our preclinical data."
Director, Cell Engineering Platform
"When building an Alzheimer's disease-related lncRNA regulatory network, the locked nucleic acid (LNA) modified probe provided by CD Biosynsis successfully broke through the non-specific binding problem in the blood-brain barrier model. The introduced quantum dot fluorescent labeling system reduces the signal to background ratio of in situ hybridization to 1:5000, laying the technical foundation for our publication in Nature Neuroscience."
Dr. Li Wei, Head, Research Group on Epigenetic Mechanisms
What DNA and RNA synthesis services can be provided?
Our services encompass custom DNA synthesis together with RNA synthesis and gene synthesis as well as oligonucleotide synthesis and modified nucleic acid synthesis. Our service offerings satisfy requirements for research purposes as well as industrial needs.
The completion time for DNA or RNA synthesis projects varies based on project details.
Project complexity and scale determine the turnaround time. Oligonucleotide synthesis takes 2-5 business days but gene synthesis and bulk RNA synthesis require 1-3 weeks.
What is the longest DNA fragment or RNA sequence that our synthesis technology can produce?
Our facility produces DNA fragments with a maximum length of 10,000 base pairs and RNA sequences that extend to several kilobases. Our advanced assembly techniques maintain both accuracy and efficiency when synthesizing longer DNA sequences.
Does this service offer modification options for both DNA and RNA molecules?
The service offers multiple DNA and RNA modifications which include fluorescent labeling, biotinylation, phosphorylation together with non-natural base incorporation. Custom modifications are also available upon request.
What verification techniques are used for quality control of synthetic DNA and RNA sequences?
The purity and integrity of our synthesized nucleic acids undergo quality control testing through mass spectrometry (MS), high-performance liquid chromatography (HPLC), and gel electrophoresis.
Can you synthesize RNA with modified nucleotides for mRNA vaccines or therapeutics?
We specialize in the synthesis of RNA molecules with modified nucleotides like pseudouridine and N1-methylpseudouridine to enhance mRNA vaccine stability and reduce immunogenicity for therapeutic uses.
Can you provide the service pricing information for DNA and RNA synthesis?
The cost for DNA and RNA synthesis services is calculated based on the length of the sequence requested and the amount needed as well as any special modifications requested and the overall project complexity. We develop pricing structures that meet your exact needs and deliver cost-effective solutions.
Are cloning and vector construction services available in addition to your DNA synthesis offerings?
Our comprehensive services support your DNA synthesis projects with cloning solutions and vector construction capabilities along with plasmid preparation for downstream applications.
Our team possesses the knowledge and resources to manage vast and high-throughput DNA/RNA synthesis projects.
Absolutely. We possess both the capacity and technical knowledge necessary to conduct large-scale and high-throughput synthesis projects while guaranteeing timely delivery combined with consistent quality for industrial and research purposes.
What types of technical support services do you provide during DNA and RNA synthesis projects?
Our technical support services cover every stage of the project from initial consultation through sequence optimization and troubleshooting until after-sales service. Our team stands ready to solve any technical issues and answer your technical questions.
In the wave of rapid development of synthetic biology, the global gene synthesis industry is undergoing unprecedented technological changes. As of 2024, the market size has reached 3.2 billion US dollars, and its growth engine comes from the innovative competition of the three giants GenScript, Integrated DNA Technologies (IDT) and Twist Bioscience. The former has pushed the accuracy of clinical-grade DNA synthesis to 99.999% with its revolutionary error correction algorithm, the middle one has reshaped the scientific research rhythm with 72-hour ultra-fast delivery, and the latter has achieved parallel production of 1.3 million oligonucleotides on a single chip with a silicon-based synthesis platform - this three-dimensional competition of "accuracy, speed, and scale" is rewriting the underlying rules of life science research.
The importance and potential of genetic research
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.